Shirley, NY – Creative Biogene, a leading innovator in RNA research solutions, is thrilled to build the next generation of sequencing-driven diagnostics and translational research solutions through native and long-read technologies, transforming genetic, epigenetic, and transcriptomic complexity into clinical products, research assays, and scalable programs designed to improve patient care. Recently, Creative Biogene announces the launch of its nanopore direct RNA sequencing service, an advanced technology that allows researchers to analyze native RNA molecules without the need for conversion to cDNA or amplification, in order to provide unprecedented insights into the transcriptome, enabling the exploration of RNA in its most natural form.
Nanopore sequencing technology provides the longest sequencing reads and, uniquely, direct RNA-sequencing. Creative Biogene offers multiple protocols for Oxford Nanopore Technologies (ONT) nanopore sequencing: Long-read and ultra-long-read DNA sequencing, targeted DNA sequencing, direct RNA sequencing, and full-length cDNA sequencing.
Our Nanopore Direct RNA Sequencing Service directly measures poly(A) tail lengths on IVT RNAs, providing insight into a key determinant of stability, translation efficiency, and overall therapeutic performance.
The ability to analyze RNA without interfering treatments opens up new avenues for understanding gene expression, splicing, and RNA modifications at a level of detail that traditional sequencing methods simply do not offer. – Marcia Brady, the marketing director of Creative Biogene
This cutting-edge service empowers researchers to conduct comprehensive characterization of transcript structures, including the identification of transcript isoforms, detailed mapping of RNA modifications, analysis of poly(A) tails, and investigation of RNA dynamics and stability. Significant applications include transcript isoform discovery, alternative splicing analysis, and RNA metabolism studies.
Standard direct RNA sequencing reads only fragments carrying a poly(A) tail, so the products of a break go uncounted and truncation gets inferred from what survives. Creative Biogene RNA QC recovers fragments regardless of tail, resolving where molecules actually broke. The service also includes a robust bioinformatics analysis pipeline tailored to meet diverse research needs, from standard transcriptome profiling to advanced custom analyses which ensures that researchers receive not just raw data, but actionable insights that can guide their investigations.
“We are committed to providing top-quality support and consultation to our clients. Our team of experts is ready to assist customers in designing their projects to achieve optimal results,” Brady added.
Creative Biogene offers HMW-DNA isolation as a service since high-quality single-molecule sequencing data depend on highest quality DNA samples. ONT has been progressing especially rapidly and is characterized by roughly annual flowcell chemistry changes and even more frequent basecaller software updates resulting in quickly improving sequence qualities.
About Creative Biogene
Creative Biogene is a pioneering service provider dedicated to advancing RNA research and technology. As a trusted partner in RNA research, Creative Biogene’s nanopore direct RNA sequencing service is designed to meet the needs of academic, clinical, and industrial researchers aiming to push the boundaries of molecular biology and genetics.
