As growing strain on pharmaceutical technology necessitates more efficient practice, Alfa Laval has launched the CultureOne
According to Bioplan Associates, single-use bioprocessing technologies now dominate the pre-commercial manufacturing space. However, Alfa Laval states many companies have not been able to make the transition to ‘full-single-use’ when harvesting proteins due to the lack of a single-use disc-stack centrifuge. The CultureOne
Alfa Laval is also working to ensure that the launch of the CultureOne
While advocacy of single-use plastic centrifuges is growing, stainless steel culture separators remain the current industry standard. According to Alfa Laval, this is limiting operational time for many pharmaceutical companies, as well as adding costs for chemicals due to the need for sterilisation between each cycle.
Plastic systems, placing a new disk stack in the centrifuge bowl each cycle, bypass the need for sterilisation and offer greater flexibility when producing new medicines – a crucial consideration in the current climate.
However, traditional single-use centrifuges have also been subject to similar limitations on operating time. Though the need for sterilisation is negated, until now, these centrifuges have only been available in batch processing formats – requiring frequent manual ejection of waste material. Efficiency is once again hampered by the necessity for internal access mid-cycle.
In response to these challenges, Alfa Laval has developed a solution that effectively addresses the shortcomings of both traditional stainless-steel and existing single-use centrifuges, achieving maximum culture yields with minimal downtime between cycles.
Moreover, unlike existing batch processing systems, the CultureOne
Each piece of the Spinsert
During each cycle, the centrifuge rotates at speeds of up to 5,000rpm, with contents experiencing up to 12,000g of force. The short settling distance and large separation area results in industry highs for biomass yields of up to 98% every cycle. Crucially, cell cultures are able to be recovered intact, allowing for later reprocessing if desired.
Minimising downtime between cycles while achieving consistently high yields has the potential to revolutionise the pharmaceutical industry. Effective application of the CultureOneIan Forrester, Business Unit Manager for Separation and Food Systems at Alfa Laval
can greatly accelerate the production of vital proteins, which form a key component of many common vaccines
“Hygiene has been propelled back under the spotlight since the pandemic started, particularly within medical environments. However, repeated sterilisation of stainless-steel equipment has a negative impact on overall lab efficiency. Going forwards, single-use, pre-sterilised Spinserts
Alfa Laval is offering a free white paper that explores the intricacies of the CultureOne’s